This study aims to investigate the relationship of workplace to family conflict, fatigue, recognition of health status and health enhancement behaviors in the subjects of middle aged businesswomen, and to analyze the factors to affect their health enhancement behaviors. The subjects of this study were 152 middle aged businesswomen from 40 to 64 years old who lived in South and North Choongchung Provinces, and their data were collected using by the survey form. The collected data were analyzed with descriptive statistics, t-test, ANOVA, Pearson’s correlation coefficients, and multiple linear regression with stepwise method. The variables with the highest influence to the health promotion activity of middle-aged working women were fatigue 16.0% (β=−.319, p<.001), family type 2.2% (β=−.163, p=.031), and work-family conflict 1.8% (β=−.181, p=.033) and it was found that these three variables explained 20.9% of health promotion activity. There needs to be plans to lower the levels of fatigue and work-family conflict that is known as predictive factors of health promotion activity of middle-aged working women as well as further social support for subjects who do not live with their family. In addition, it is required to establish the simple exercise facilities in the workplace so as for the middle aged businesswomen who hardly spend the time to enhance their health so often. Moreover, the atmosphere of the workplace should be organized to increase the perception and the practices on the health enhancement by sports for all that can be simply practiced in the workplace without specific facilities.

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