The purpose of this study was to develop the mobile office stress scale and examine the negative effects of mobile office stress on work-family conflicts and work-leisure conflicts. In addition, moderating effects of relation oriented organizatonal culture on relationships between mobile office stress and work-family conflict and work-leisure conflict were examined. Data were obtained from employees using mobile office in workplace, and total 249 answers were used for analysis. For the mobile office stress scale, a total of 13 items, consisting of 4 factors were developed. The results of structure equation modeling analysis showed that mobile office stress had significant effects on work-family conflicts and work-leisure conflicts. Also the results of hierarchial regression analyses showed that relation oriented organizational culture did not have a significant moderating effect for the relationship between mobile office stress and work-family conflict. However, for the relationship between mobile office stress and work-leisure conflict, relation oriented organizational culture had a significant moderating effect. Finally, implications, limitations, and suggestions and directions for future research were discussed.