This study is a randomized control trial (RCT) to compare the effects of the electrocardiogram waveform simulation-based Healing Rhythm System on stress, electroencephalography (EEG), heart rate, and anxiety on healthy college students. Healthy college students without any disease were recruited. A total of 68 participants were collected and analyzed in 31 experimental groups, 17 placebo group, and 20 control group.

The analysis method was repeated measurement of ANOVA and it was conducted 8 times in total. The effect measurement was checked before and after exposure to stressors and six times every five minutes after intervention.

The results of the study showed that stress was significantly different in the experimental group at 10 minutes (F=4.33, p=.017) and 15 minutes after the intervention, and the EEG was significantly different in the experimental group at the relative alpha Fp1, F3 (F=5.753, p=.005) after the intervention, and the heart rate was significantly different in the experimental group at 15 minutes and 20 minutes (F=3.586, p=.033). The anxiety was significantly different in the experimental group after the pre and post-treatment of stressors (F=18.609, p<.001).

Therefore, HR system will be widely used as a program to induce calming to healthy university students in stressful situations.